Note

Chemical Synthesis and Characterization of J46 Peptide, an Atypical Class IIa Bacteriocin from Lactococcus lactis subsp. cremoris J46 Strain

Samar Lasta, Ziad Fajloun, Hervé Darbon, Pascal Mansuelle, Nicolas Andreotti, Jean-Marc Sabatier, Abdellatif Boudabous, François Sampieri

Received: November 22, 2007 / Accepted: January 30, 2008
© Japan Antibiotics Research Association

Abstract Bacteriocin J46 is a 27-residue polypeptide produced by Lactococcus lactis subsp. cremoris J46 in fermented milk. The natural form of J46 (nJ46) exhibits a broad antimicrobial spectrum. Herein, we produced the synthetic form of J46 (sJ46) by solid-phase chemical synthesis. The biochemical and physico-chemical properties of sJ46, as well as its antimicrobial activity, were found to be identical to those of its natural counterpart nJ46. It showed a potent antimicrobial activity against both lactic acid bacteria and other Gram-positive microorganisms. 1H-NMR conformational analysis of sJ46 indicates that it adopts a flexible random coil structure.

Keywords bacteriocin J46, antimicrobial activity, synthetic peptide, conformational analysis, Lactococcus lactis subsp. cremoris J46


S. Lasta, P. Mansuelle: Laboratoire de Biochimie, CNRS FRE 2738, Faculté de Médecine Nord, Bd Pierre Dramard, 13916 Marseille Cedex 20, France
Z. Fajloun: Laboratoire Cellpep S.A, 13-15 Rue Ledru-Rollin, 13015 Marseille, France, Present address: Université Libanaise, Faculté des Sciences, Section III, Tripoli, Lebanon
H. Darbon: AFMB UMR6098, Parc Scientifique et Technologique de Luminy Case 932, 163 Avenue de Luminy, 13288 Marseille Cedex 09, France
F. Sampieri (Corresponding author), N. Andreotti, J.-M. Sabatier: Laboratoire ERT 62 «Ingénierie des peptides à visée théapeutique», Université de la Méditerranée-Ambrilia Biopharma S.A, Faculté de Médecine Nord-Bd Pierre Dramard, 13916 Marseille Cedex 20, France, E-mail: francois.sampieri@univmed.fr
S. Lasta, A. Boudabous: Laboratoire Microorganismes et Bio-molécules Actives, Département de Biologie, Faculté des Sciences de Tunis, Université El Manar, 2092, Tunis, Tunisia