Cylindrocyclin A, a New Cytotoxic Cyclopeptide from Cylindrocarpon sp.

Daniela Weber, Gilda Erosa, Olov Sterner, Timm Anke

Dedicated to the memory of Professor Kenneth L. Rinehart.

Received: May 30, 2006 / Accepted: August 11, 2006
© Japan Antibiotics Research Association

Abstract In the course of a screening of fungal extracts for new metabolites with cytotoxic activities cylindrocyclin A (1) was isolated. The producing strain was identified as Cylindrocarpon sp. by microscopy and ITS rDNA sequence analysis. 1 is a novel compound that exhibits cytotoxic acticity against six different cell lines with IC50 values ranging from 11 to 53 μM. 1 has no antibacterial or antifungal activity. The compound is a cyclic nonapeptide comprising three alanines, five leucines and one isoleucine. Four amino acids are N-methylated. Its structure was elucidated by spectroscopic methods.

Keywords new metabolite, cyclopeptide, cytotoxic, cylindrocarpon

T. Anke (Corresponding author), D. Weber: Institute of Biotechnology and Drug Research, Erwin-Schrödinger-Str. 56, D-67663 Kaiserslautern, Germany,
E-mail: timm.anke@ibwf.uni-kl.de
G. Erosa, O. Sterner: Division of Organic Chemistry, Lund University, P.O.B. 124, S-22100 Lund, Sweden