NOTE  

Biosynthesis of 2-Deoxystreptamine-containing Antibiotics in Streptoalloteichus hindustanus JCM 3268: Characterization of 2-Deoxy-scyllo-inosose Synthase

Toshifumi Hirayama, Hideyuki Tamegai, Fumitaka Kudo, Kazumasa Kojima, Katsumi Kakinuma, Tadashi Eguchi

Received: February 20, 2006 / Accepted: May 24, 2006
© Japan Antibiotics Research Association

Abstract A part of the new biosynthetic gene cluster for 2-deoxystreptamine-containing antibiotics was identified from Streptoalloteichus hindustanus. The alloH gene in the gene cluster was deduced to encode 2-deoxy-scyllo-inosose synthase and the expressed protein AlloH was confirmed to have this enzyme activity. Furthermore, biochemical properties of AlloH were studied.

Keywords 2-deoxystreptamine biosynthetic genes, Streptoalloteichus hindustanus, 2-deoxy-scyllo-inosose synthase, kinetics


T. Eguchi (Corresponding author), T. Hirayama: Department of Chemistry and Materials Science, Tokyo Institute of Technology, 2-12-1 O-okayama, Meguro-ku, Tokyo 152-8551, Japan,
E-mail: eguchi@cms.titech.ac.jp
H. Tamegai, F. Kudo, K. Kojima, K. Kakinuma: Department of Chemistry, Tokyo Institute of Technology, 2-12-1 O-okayama, Meguro-ku, Tokyo 152-8551, Japan
Present address: Department of Chemistry, College of Humanities and Sciences, Nihon University, 3-25-40 Sakurajosui, Setagaya-ku, Tokyo 156-8550, Japan