Trends of Arbekacin-resistant MRSA Strains in Japanese Hospitals (1979 to 2000)

Naofumi Tsuchizaki, Keiko Ishino, Fumiko Saito, Jun Ishikawa, Mutsuyasu Nakajima, Kunimoto Hotta

Received: February 10, 2006 / Accepted: April 7, 2006
© Japan Antibiotics Research Association

Abstract A total of 472 clinical strains of methicillin-resistant Staphylococcus aureus (MRSA) isolated in Japan between 1979 and 2000 were investigated for resistance to 8 aminoglycosides, 4 aminoglycoside-modifying enzyme gene profiles, and AluI-restriction fragment length polymorphism of the coagulase gene determined by polymerase chain reaction assay. The majority of MRSA strains tested belonged to 4 groups based on coa-RFLP: L21, L22, L31, and M22. About 90% of recent isolates belonged to type L21, indicating the spread of a specific type of MRSA in Japan. Of the type L21 strains, 41.9% included the aac(6´)/aph(2´´) gene, which was one of the risk factors of arbekacin (ABK) resistance, but only 5.5% were resistant to ABK. In contrast, all of the type M22 strains carried aac(6´)/aph(2´´) and 70.1% showed ABK resistance. Among the other types, less than 20% of strains showed ABK resistance. These results suggested that ABK has maintained potent activity. If the predominance of type L21 continues, there will be no progression to ABK resistance in MRSA. However, it may be necessary to monitor the trends in type M22 continuously.

Keywords MRSA, arbekacin resistance, coa-RFLP typing

K. Ishino (Corresponding author), F. Saito, J. Ishikawa, K. Hotta: Department of Bioactive Molecules, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo 162-8640, Japan, E-mail: ishino@nih.go.jp
N. Tsuchizaki: Japan Microbiological Clinic, Co., Ltd., 5-17-1, Okada, Atsugi, Kanagawa 243-0021, Japan
M. Nakajima: Department of Applied Biological Science, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan